Driven, focused, and hard-working individual with a Ph.D. in Biochemistry & Molecular Biology and a significant technical acumen. Experienced in performing complex laboratory functions, providing individualized training to others, and fostering a positive and efficient working environment. Equipped with a proficiency in several technical competencies in addition to a keen understanding of biochemistry and molecular biology best practices.

Ph.D., Biochemistry & Molecular Biology

Baylor College of Medicine, Houston, TX (Currently Pursuing)

2015 APS/CCP4Crystallography Summer School, Argonne National Laboratory

Awards Include: American Society of Microbiology 2015 Outstanding Poster Award (Top 2%), American Society of Microbiology 2015 Travel Award, and Molecular Basis of Infectious Disease Fellowship

B.S., Biochemistry and Molecular Biology, State University of New York at Albany, Albany, NY

A.S., Biological Sciences, Hudson Valley Community College, Troy, NY

Baylor College of Medicine, Houston, TX, July 2012 to March 2016

Protein Biochemist, Ph.D. Candidate

  Directed a variety of technical operations within a fast-paced environment with a focus on increasing efficiency and productivity levels

  Utilized superior technical acumen in order to develop a periplasmic-wide protein complementation assay which required using a library holding over 600 ORFs

  Maintained a keen knowledge of designing purification and expression protocols for an extensive number of proteins in addition to overseeing the classification and optimization of binding affinities and stability for 10 proteins

  Performed high throughput fragment based screens for serine beta-lactamase inhibitors along with providing individualized training/coaching to high school, undergraduate, and graduate students

  Fostered positive working relationships with co-workers which included medical professionals, Scientists, and graduate students

PCR, Gibson Assembly, Site-Directed Mutagenesis, Bacterial Protein Expression and Purification, Protein Stability (CD and Thermoflour), Protein Crystallography (MR and SAD-Phasing), Protein Binding, BIACORE, ITC, AKTA Avant and Explorer, HPLC, Systems, FPLC Systems, Conventional and Affinity Chromatography, Protein Gel Assays (SDS-PAGE) Coomassie, Silver Staining, Enzymatic Fluorescence Assays, Colorimetric Assays, ELISA, and Western Blot

  PCR, Gibson Assembly, Site-Directed Mutagenesis, Bacterial Protein Expression and Purification, Stojanoski V., Chow D.-C., Hu L., Sankaran B., Gilbert H., Prasad B.V.V., and Palzkill T. (2015) A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis, J Biol Chem 2015 Apr 17;290(16):10382-94.

  Stojanoski V.,Chow D.-C., Fryszczn, B.G., Hu L., Nordmann P., Poirel L., Sankaran B., Prasad B.V.V., and Palzkill T. (2015) Structural basis for different substrate profiles of two closely related class D beta-lactamases and their inhibition by halogens, Biochem 2015 Jun 2;54(21):3370-80.

  Stojanoski V., Adamski C.J., Mehta S.C., Sankaran S., and Palzkill T.G. The absence of the active site nucleophile increases the thermodynamic stability of serine β-lactamases. Submitted to Biochemistry.

  Stojanoski V., Sankaran B., and Palzkill T.G. Halide ions facilitate decarboxylation of the general base in class D β-lactamases crystallography and molecular dynamics studies. In preparation estimated submission date February 2016

  Stojanoski V., Hu L., Sankaran B., Prasad B.V.V., and Palzkill T. Molecular basis of the different substrate specificity of two members from the OXA-48-like family of class D β-lactamases. In preparation estimated submission date February 2016.