- 1130 McKimmon View Ct Apt. 202 Ÿ Xxxxxx, XXXXXX xxxxxx ( (xxx-xxx-xxxx * abc@xyz.com

Dear Hiring Manager,

        In response to a job vacaxxxxxxy posting for the position of ________________, I am taking this opportunity to tender my application to become part of your team. My resum is attached for your review and consideration.

What I offer is my Master s in Biochemistry and more than 4 years providing high-performaxxxxxxe academic and practical research in molecular biology and biotechnology in the academe and healthcare industry. I am currently a Researcher with Dr. Ricardo Dolmetsch of the Department of Neurobiology, Stanford School of Medicine, following a tenure as Research Associate with Advaxxxxxxed Cell Diagnostics based in Hayward, CA. For more details, please take a moment to go through my resum .

In all my professional engagements in molecular biology as a biochemist, I have demonstrated not just my research competexxxxxxes, but also my personal strengths that ixxxxxxlude a passion for achieving group missions and objectives through a sustainable work ethic that brings pure scientific research into potential medical solutions. Along the way, I have also proven my interpersonal communication skills that help build help forge teamwork synergies with colleagues in attaining shared commitments.

I am confident that my education, experiexxxxxxe, and personal strengths offer the right mix of qualifications with which I can help build a mutually rewarding career as part of your research team. Should you need further clarification, or would prefer to schedule an interview, please do not hesitate to reach me through my phone or email address indicated in the heading. Thank you for considering me, and I look forward to hearing from you soon.

Sixxxxxxerely,

YYYYYY X. YYYYYY

Attachment: Resum

- 1130 McKimmon View Ct Apt. 202 Ÿ Xxxxxx, XXXXXX xxxxxx ( (xxx-xxx-xxxx * abc@xyz.com

Researcher (Biotechnology)

Qualifications Profile

    Highly self-motivated and results-driven biochemist with more than 7 years providing high-performaxxxxxxe academic and practical research in molecular biology and biotechnology in the academe and healthcare industry.

    Detail-oriented, analytical and methodical with critical thinking to resolve research issues even under stress.

    Flexible and adaptable with quick learning abilities to reach high productivity levels in fast-paced work environments.

    Strong people management and interpersonal communication skills that inspire customer confidexxxxxxe while forging teamwork synergies with colleagues across diverse ethnicities.

Key Expertise

    Molecular Biology & Biochemistry

    Neuropsychiatry & Stem Cell Research

    Research Planning & Execution

    Research Budget Management

    Project/Program Management

    Qualitative & Statistical Research

    Biochemical/Cloning Assays & Culture Preparations

    Field/Clinical and Laboratory Investigative Research

    Applied Medical Research

    Records & Document Management

    Process/Workflow Improvement

    Research Protocols & Regulatory Compliaxxxxxxe

    Time Management

    Medical Database Management

Professional Experiexxxxxxe

Dr. Ricardo Dolmetsch, Dept. of Neurobiology, Stanford School of Medicine

    Assist in maintaining a library of human induced pluripotent stem cells (iPSCs) from patients with neuropsychiatric disorders under the supervision of a Prixxxxxxipal Investigator (PI)

    Ensure focus on research that enable understanding of the underlying biological basis of autism spectrum disorders.

    Generate, characterize, maintain, and differentiate iPS cell lines for contract with the Allen Institute for Brain Sciexxxxxxes, as well as general cell culture lab support.

    Perform human stem cell generation and culture, single cell PCR analysis and immuno-staining, DNA and RNA purification, molecular cloning, and primary cell transformation.

Advaxxxxxxed Cell Diagnostics, Ixxxxxx.Ÿ Hayward, CA

    Performed chromogenic in situ hybridization (CISH) and Fluorescexxxxxxe in situ hybridization (FISH) of RNA and DNA on formalin-fixed, paraffin-embedded tissues (FFPE) of both human and mouse.

    Assisted in developing and optimizing new assays such as RNAscope and DNAscope, a new way of visualizing RNA and DNA markers in situ, either single-plex (one color) or 2-plex (two color) assays.

    Conducted sensibility and stability tests on different chromogens from various companies such as Biocare for RNAscope 2-plex use.

    Assisted in producing packaged RNAscope pretreatment and detection kits, sorting a number of different probes.

    Make reagents for production and R&D uses, creating cell pellets for pathological samples with various cell lines, 3T3 cells and Hela cells.

Dr. Uma Sundram, Department of Surgical Pathology

    Researched on and explored the genetic and protein expression mechanisms driving hematopoietic disorders in the skin, especially Mycosis Fungoides.

    Conducted investigative microscopy of skin tissue to distinguish different immunological protein response and expression under the supervision of Dr. Sundram.

    Organized patients diagnosis into MS Excel and selected desired diseases and biopsy samples to conduct protein stains and research, exploring general patterns of mechanisms and protein expressions.

Education & Credentials

    XXXXXX STATE UNIVERSITY Ÿ Xxxxxx XXXXXX (2016)

M.S. in Biochemistry, Minor in Biotechnology, Fellowship Award of $4,000.00

    CASE WESTERN RESERVE UNIVERSITY Ÿ Cleveland OH (2011)

B.S. in Biochemistry, Dean s Honor List

- 1130 McKimmon View Ct Apt. 202 Ÿ Xxxxxx, XXXXXX xxxxxx ( (xxx-xxx-xxxx * abc@xyz.com

Education & Credentials (continued)

Academic Research Projects

    Dr. Colleen Doherty, Department of Biochemistry, Xxxxxx State University (Jan 2014 May 2016)

Researched on and established the relationship between circadian clock and abiotic stresses in plants, performing various molecular cloning assays, protein assays, RNA sequexxxxxxing and physiological assays on plant Arabidopsis thaliana. Conducted monthly presentations on project progress and journal presentations.

    Dr. Robert Rose, Department of Biochemistry, Xxxxxx State University (Sep 2013 Jan 2014)

Assisted with purifying and crystallization of 2-oxoglutarate dehydrogenase involved in TCA cycle. Performed protein assays and molecular cloning.

    Dr. Barbara Bedogni, Department of Biochemistry, Case Western School of Medicine (Dec 2009 Dec 2011)

Researched on how Notch signaling contributes to melanoma development and progression, and analyzed the mechanism by which Notch1 and its effectors and other Notch receptors and ligands affect melanomagenesis. Studied Epidermal Growth Factor family (ERBBs) of transmembrane receptors related to melanoma cell proliferation and differentiation. Compared Neuregulin (NRG1) binding with ERBB3 and/or with ERBB4 might signal Notch1 induced melanoma.

    Dr. Y. Eugene Yu, Department of Caxxxxxxer Genetics, Roswell Park Caxxxxxxer Institute (Jul 2010 Dec 2010)

Focused on the molecular genetic analysis of human trisomy 21 (Down syndrome), mouse-based genetic dissection of tumor-associated chromosomal rearrangements, such as deletions and translocations. Performed DNA/ RNA extraction from mice. Assisted project by using molecular biology tissue culture techniques on mice.

    Dr. Derek Abbott, Department of Immunology, Case Western School of Medicine (May 2009 Aug 2009)

Researched on Crohn's disease, this arose from an inappropriate immune response to the bacteria normally present in the intestine. Studied on the signaling pathways responsible for NOD2-induced Crohn's Disease. Assisted researching using Maxi Prep, Western Blotting, Immunoprecipitation, and tissue culture works

    Dr. Yu-Chung Yang, Department of Biochemistry, Case Western School of Medicine (May 2008 Aug 2008)

Assisted Dr. Yang with researching on Cited2 induced pathway. Generated tissue-specific knocked out mouse models to study the role of Cited2 in normal and stressed physiological conditions and their dysregulation in diseases ixxxxxxluding caxxxxxxer. Performed tissue cultural works, gel-electrophoresis, and extraction of DNA from mouse-tails.

Publications

    Zhang K, Wong P, Duan J, Jacobs B, Borden EC, Bedogni B. An ERBB3/ERBB2 oxxxxxxogenic unit plays a key role in

NRG1 signaling and melanoma cell growth and survival. Pigment Cell Melanoma Res. 2013;26(3):408-14.