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Yyyyyy x. yyyyyy
14345 Saratoga Ave., #0000 xxxxxx xxxx , xxxx , xxxxx 00000 ● (xxx-xxx-xxxx ● abc@xyz.com
Molecular Scientist
Molecular Biology ~ Cell Biology ~ Technical Writing ~ Assay Development ~ DNA Sequencing
Immunology ~ ELISAs ~ Toxicology Testing ~ Laboratory Operations ~ Quality Assurance & Control
Profile
Proficiency in immunology, molecular diagnostics, ELISA development, gene subtraction, cDNA microarrays, PCR application, cDNA cloning, DNA/RNA/protein analysis, and RNase protection assay development.
Sound abilities in thrombin generation, Western blotting, cell-based assays, cytometry testing, and photo optic/chromogenic/fluorescent testing.
Adept at DNA sequence analysis (sequencer, blast search, DNA strider) and using instrumentation and methods spanning ABI Sequencer, BioRobotic Arrayer, GC (fatty acid composition analysis), HPLC (retinoid acid isomer separation).
Advanced ability to balance concurrent project tasks in alignment with timelines and budgets.
Solid experience in steering regulatory compliance, administering quality control to ensure accuracy of data, and maintaining state-of-the-art lab instruments and equipment.
Meticulously document procedures, calculations, measurements, observations, and analysis of results.
Polished communication and interpersonal skills as evidenced by talents in teaching, mentoring, and public speaking.
Expertise at authoring SOPs, safety procedures, journal publications, brochures, and user manuals.
Well-versed in lab safety regulations including hazardous materials management and OSHA compliance.
Skilled in using word processing, spreadsheets, graphical and presentation software applications.
Professional Experience
Alpha Biolaboratory, Inc., 2004 to Present
Research Scientist
Spearhead all aspects of custom DNA sequencing and genetic testing services from hands-on test administration to supervising junior personnel.
Facilitate enlightening genetic laboratory workshops for high school students and college interns.
Achieve and maintain first-rate quality results by standardizing procedures for specialty genetic testing and high-throughput sequencing services such as Illumina de novo, transcriptome, and methylation sequencing.
Reliably maintain complete and accurate laboratory documentation and electronic files.
Drive company revenues by commercializing, promoting, and demonstrating DNA testing kit for genetic laboratories such as Red Hair test and Bitter Taster Detector.
Zytogene, Inc., 2002 to 2004
Research Scientist
Supported R&D department by assembling subtraction libraries for high-throughput immunogen screening.
Established protocols and SOPs to optimize quality and consistency throughout research projects.
Effectively devised methods, techniques, and evaluation criteria for obtaining results.
Continued
Yyyyyy x. yyyyyy Page 2 of 2 Professional Experience continued
Genemed Biotechnologies, Inc., 2001 to 2004
Radiation Safety Officer
Diligently enforced radiation safety throughout corporation by delivering in-depth safety training to staff and preparing comprehensive safety manuals.
Ordered radioactive substances to support company operations and controlled associated inventory.
Maintained up-to-date licensure and critical record-keeping systems for state safety inspection.
Associate Research Scientist
As an integral member of R&D department, isolated liver cancer-related oncogenes by coupling full-length low abundant gene subtraction and DNA microarrays
Consistently ensured strict adherence to safety and quality standards such as GLP and OSHA guidelines.
Faculty of Toxicology, Texas A&M University, 1994 to 2001
Postdoctoral Research Associate
Resourcefully employed subtractive hybridization and DNA microarrays to investigate gene regulation mediated by estrogen and antiestrogens in breast cancer cells.
Graduate Research Assistant
Studied aryl hydrocarbon receptor-mediated antiestrogenic activity in human breast cancer cells and accurately documented findings.
Education
Ph.D., Toxicology-Cancer Biology, Texas A&M University, College Station, TX, 1999
M.S., Food Science and Human Nutrition, University of Florida, Gainesville, FL, 1993
B.S., Medical Technology, China Medical University, Taichung, Taiwan, 1990
Academic Honors & Recognition:
Distinguished Graduate Student Doctoral Research Award, Assoc. of Former Students, Texas A&M University
1st Place, SOT in Vitro Specialty Presentation, Society of Toxicology
George T. Edds Award, Outstanding Graduate Student in Toxicology
Software
Microsoft Excel, Adobe Photoshop, Microsoft PowerPoint, Microsoft Word
Community Involvement
Lynbrook Job Shadow and Summer Internship Program, 2008 to 2013
Instructor
Taught molecular biology featuring hands-on lab activities such as DNA extraction, PCR amplification, gel electrophoresis, and genotyping to students who were subsequently admitted into the nation's top universities (i.e. University of California at Berkeley and California Institute of Technology).
Yyyyyy x. yyyyyy
14345 Saratoga Ave., #0000 xxxxxx xxxx , xxxx , xxxxx 00000 ● (xxx-xxx-xxxx ● abc@xyz.com
Addendum
Publications
Chen, I., Hsieh, T., Thomas, T., and Safe, S. 2001. Mechanisms of inhibitory aryl hydrocarbon receptor-estrogen receptor crosstalk in MCF-7 breast cancer cells: identification of genes downregulated by AhR agonists using suppression subtractive hybridization. Gene 262: 207-214
Chen, I., McDougal, A., Wang, F., and Safe, S. 1998. Aryl hydrocarbon receptor-mediated antiestrogenic and antitumorigenic activity of diinolylmethane. Carcinogenesis 19(9): 1631-1639. 51(8): 1069-1076
Ramamoorthy, K., Wang, F., Chen, I., Norris, J.D., McDonnell, D.P., Gaido, K.W., Bocchinfuso,W.P., Korach, K.S., and Safe, S. 1997. Potency of combined estrogenic pesticides. Science 275: 405-406
Chen, I., Safe S., and Bjeldanes, L. 1996. Indole-3-carbinol and diindolylmethane as aryl hydrocarbon (Ah) receptor agonists and antagonists in T47D human breast cancer cells. Biochem. Pharmacol. 51(8): 1069-1076
Chen, I C., Chapman, F A., Wei, C I., and O Keefe, S F. 1996. Preliminary studies on SDS-PAGE and isoeletric focusing identification of sturgeon sources of caviar. J. Food Sci. 61(3): 533-535, 539
Chen, I-C., Chapman, F A., Wei, C-I., Portier, K M., and O Keefe, S F. 1995. Differentiation of cultured and wild sturgeon (Acipenser oxyrinchus desotoi) base on fatty acid compositions. J. Food Sci. 60(3): 631-635
Samudio, I., Vyhlidal, C., Wang, F., Stoner, M., Chen, I., Kladde, M., Barhoumi, R., Burghardt, R. and Safe, S. 2001. Transcriptional activation of deoxyribonucleic acid polymerase gene expression in MCF-7 cells by 17-estradiol. Endocrinology, 142: 1000-1008
Xie, W., Duan, R., Chen, I., Samudio, I. and Safe, S. 2000. Transcriptional activation of thymidylate synthase by 17-estradiol in MCF-7 human breast cancer cells. Endocrinology, 141: 2439-2449
Gaido, K., Dohme, L., Wang, F., Chen, I., Blankvoort, B., Ramamoorthy, K., and Safe, S. 1998. Comparative estrogenic activity of wine extracts and organochlorine pesticide residues in food. Environ. Health Persp. 106: 1347-1351
Ramamoorthy, K., Wang, F., Chen, I., Norris, J.D., McDonnell, D.P., Gaido, K.W., Bocchinfuso, W.P., Korach, K.S., and Safe, S. 1997. Estrogenic activity of a dieldrin/toxaphene mixture in the mouse uterus, MCF-7 human breast cancer cells and yeast-based estrogen receptor assays: no apparent synergism. Endocrinology 138: 1520-1527
Fielden, M.R., Chen, I., Chittim, B., Safe, S.H., and Zacharewski, T. 1997. Examination of the estrogenicity of 2,4,6,2 ,6 -pentachloro-4-biphenylol (HO-PCB 104), and a further chlorinatedderivative, 2,4,6,2 ,4 ,6 -hexachlorobiphenyl (PCB 155). Environ. Health Persp. 105: 1238-1248
Moore, M., Mustain, M., Daniel, K., Chen, I., Safe, S., Zacharewski, T., Gillesby, B., Joyeux, A., and Balaguer, P. 1997. Antiestrogenic activity of hydroxylated polychlorinated biphenyl congeners identified in human serum. Toxicol. Appl. Pharm. 142: 160-168
Connor, K., Ramamoorthy, K., Moore, M., Mustain, M., Chen, I., Safe, S., Zacharewski, T., Gillesby, B., Joyeux, A., and Balaguer, P. 1997. Hydroxylated polychlorinated biphenyls (PCBs) as estrogens and antiestrogens: structure-activity relationships. Toxicol. Appl. Pharm. 145: 111-123
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Ruh, M F., Zacharewski, T., Connor, K., Howell, J., Chen, I., and Safe, S. 1996. Naringenin: a weakly estrogenic bioflavonoid that exhibits antiestrogenic activity. Biochem. Pharmacol. 50(9): 1485-1493
Connor, K., Howell, J., Chen, I., Liu, H., Berhane, K., Sciarretta, C., Safe, S., and Zacharewski, T. 1996. Failure of chloro-s-triazine-derived compounds to induce estrogen receptor-mediated responses in vivo and in vitro. Fund. Appl. Toxicol. 30(1): 93-101
O Keefe, S., Gaskins-Wright, S., Wiley, V., and Chen, I. 1994. Levels of trans geometrical isomers of essential fatty acids in some unhydrogenated U.S. vegetable oils. J. Food Lipids 1: 165
Lin., C Y., Chen, I C., Cheng, T I., Liu, W T., Hwang, B., and Chiang, B N. 1992. Virus-like particles with reverse transcriptase activity associated with Kawasaki disease. J. Med. Virol. 38: 175-182
Articles in Non-refereed Journals:
Chen, I. and Safe, S. 1997. Estrogen- and growth factor-induced proliferation of MCF-7 cells: inhibition by 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD). Organochlorine Compounds 34: 338-341
Chen, I., Harper, N., and Safe, S. 1995. Inhibition of TCDD-induced responses in B6C3F1 mice and hepa 1c1c7 cells by indole-3-carbinol. Organochlorine Compounds 25: 57-60
Gaido, K., Ramamoorthy, K., Wang, F., Chen, I-C., Norris, J.D., McDonnell, D.P., Bocchinfuso, W.P., Korach, K.S., and Safe, S. 1998. Activity of weakly estrogenic mixtures in the mouse uterus, MCF-7 cells, and yeast-based estrogen receptor assays: lack of synergism. Eisenbrand, G., Hannelore, D., Elias, P.S., Dayan, A.D., Grunow, W., Kemper, F.H., Loser, E., Metzler, M., Schlatter, J. (eds.), Wiley-VCH, DFG, 232-239
Safe, S., Connor, K., Ramamoorthy, K., Moore, M., Wang, F., Mustain, M., Chen, I., Zacharewski, T., Gillesby, B., Joyeaux, A., and Balaguer, P., Gaido, K., Leonard, L., Maness, S.C., and McDonnell, D.P. 1998. Estrogenic activity of hydroxylated polychlorinated biphenyls (PCBs) and their interactions. Eisenbrand, G., Hannelore, D., Elias, P.S., Dayan, A.D., Grunow, W., Kemper, F.H., Loser, E., Metzler, M., Schlatter, J. (eds.), Wiley-VCH, DFG, 200-207
Safe, S., Connor, K., Ramamoorthy, K., Moore, M., Wang, F., Mustain, M., Chen, I., Zacharewski, T., Gillesby, B., Joyeaux, A., and Balaguer, P., Gaido, K., Leonard, L., Maness, S.C., and McDonnell, D.P. 1997. Estrogenic activity of hydroxylated polychlorinated biphenyls (PCBs) and their interactions. In: Hormonal Toxicants in Food, Proceedings.
Safe, S., Chen, I., and Porter, W. 1996. Dietary indoles with antiestrogenic activity in common vegetables and their implication. In: Estrogen, Progestins and Their Antagonists (E. Pavlik, ed.), Birkhouser, Boston. pp.73-97
Presentations
Chen, I., Hsieh, T., Thomas, T., and Safe, S. 2000. Identification of estrogen-induced genes down-regulated by 2,3,7,8- tetrachlorodibenzo-p-dioxin (TCDD) by coupling suppression subtractive hybridization and cDNA microarrays. Toxicologist (Abstr 1803)
Chen, I. and Safe., S. 1999. Identification of estrogen-induced genes downregulated by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) by suppression subtractive hybridization. Toxicologist 48(1-S): 214 (Abstr 1007)
Chen, I., McDoougal A., and Safe, S. 1998. Antiestrogenic and antitumorigenic activity of diindolyl-methane is mediated through the Ah receptor. Toxicologist 42(1): 387 (Abstr: 1908)
Chen, I. and Safe S. 1997. Antiestrogenic activities of indole-3-carbinol, diindolylmethane and substituted analogs in MCF-7 human breast cancer cells. Toxicologist 36(1): 236 (Abstr 1197)
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Chen, I., Safe S., and Bjeldanes, L. 1996. Indole-3-carbinol and diindolylmethane as aryl hydrocarbon (Ah) receptor agonists and antagonists in T47D human breast cancer cells. Toxicologist 30(1): 183 (Abstr 934)
Chen, I., Liu, H., and Safe, S. 1995. Interactive effects of indol-3-carbinol (I3C) and 2,3,7,8tetrachlorodibenzo-p-dioxin (TCDD) in T47D human breast cancer cells: I3C inhibits induction of CYP1A1 by TCDD. Toxicologist 15(1): 237 (Abstr 1271)
Chen, I-C., Chapman, F A., Wei, C-I., Portier, K M., and O Keefe, S F. 1994. Differentiation of cultured and wild sturgeon (Acipenser oxyrinchus desotoi) based on fatty acid composition. Institute of Food Technologist Annual Meeting
Chen, I-C., Chapman, F A., Wei, C-I., Portier, K M., and O Keefe, S F. 1993. Identification of cultured and wild Gulf sturgeon (Acipenser oxyrinchus desotoi) based on fatty acid composition. Third Joint Atlantic Fisheries Technology Society and Tropical and Subtropical Fisheries Technology Society Conference
Safe, S., McDougal, A., Chen, I., Ramamoorthy, K., and Sethi-Gupta, M. 1999. Antiestrogenic and antitumorigenic activities of diindolylmethane. Toxicologist 48(1-S): 105 (Abstr 490)
Blankvoort, B., Gaido, K., Leonard, L., Wang, F., Chen, I., Ramamoorthy, K., and Safe, S. 1998. Comparative estrogenic activity of organochlorine pesticide residues in food and wine extracts. Toxicologist 42(1): 164 (Abstr 811)
Safe, S., Ramamoorthy, K., Wang, F., Chen, I., Norris, J.D., McDonnell, D.P., Gaido, K.W., Bocchinfuso, W.P., and Korach, K.S. 1997. Estrogenic activity of a dieldrin/toxaphene mixture in the mouse uterus, MCF-7 human breast cancer cells and yeast-based estrogen receptor assays: no apparent synergism. Toxicologist 36(1): 294 (Abstr 1497)
Ramamoorthy, K., Wang, F., Chen, I., Maness, S.C, Leonard, L., Gaido, K., and Safe, S. 1997. Estrogenic activities of 2 ,3 ,4 ,5 -tetrachloro- and 2 ,4 ,6 -trichloro-4- biphenylol: no synergistic interactions. Toxicologist 36(1): 295 (Abstr 1498)
Gould, J.C., Leonard, L., McDonnell, D.P., Connor, K., Chen, I., Zacharewski, T., Safe, S, and Gaido, K.W. 1997. Bisphenol A interacts with the estrogen receptor in a distinct manner from estradiol. Toxicologist 36(1): 295 (Abstr 1501)
Zacharewski, T., Connor, K., Howell, J., Chen, I, Liu, H., Berhane, K., Sciarretta, C., and Safe, S. 1996. Failure of chloro-s-triazine-derived chemicals to induce estrogen receptor-mediated resoinses in vivo and in vitro. Toxicologist 30(1): 144 (Abstr 735)
Connor, K., Howell, J., Chen, I., Liu, H., Berhane, K., Sciarretta, C., Safe, S., and Zacharewski, T. 1996. Failure of chloro-s-triazine-derived compounds to induce estrogen receptor-mediated responses in vivo and in vitro. Amer. Chem. Soc. 211(1-2)
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